Fig. 7
Evaluation of the effect of 9 on cell cycle regulation and RNA synthesis. a Gating strategy. b Results of the cell cycle analysis at different concentrations of compound 9. Results of the cell cycle analysis (2N, S phase, 4N) expressed in total cell numbers (c, n = 2) and relative cell numbers (d, n = 2). e Total RNA yield from trypanosomes exposed to different concentrations of compound 9 (n = 2). RNA content quantified using RT-qPCR specifically targeting SL-RNA (f, n = 2 with two replicates), 18S rRNA (g, n = 2 with two replicates) and TERT mRNA (h, n = 2 with two replicates). RNA content and transcript levels are expressed as relative to the non-treated trypanosomes (control). Error bars are SEM. Source data are provided as a Source Data file.
