Table 1 Genetic specific primers for single cell RT-PCR.
From: TRPV1 activity and substance P release are required for corneal cold nociception
Gene | Forward Primer | Reverse Primer | Anneal | Amplicon Size (bp) |
|---|---|---|---|---|
Trpv1 | ACCACGGCTGCTTACTATCG | CGGAAATAGTCCCCAACGGT | 52 °C | 77 |
Tac1 | CGGCCAAGGAGAGCAAAGA | ACGGCCACGAGGATTTTCAT | 62 °C | 96 |
Gapdh | CCCAGCAAGGACACTGAGCAA | TTATGGGGGTCTGGGATGGAAA | 65 °C | 93 |
