Fig. 1: Oxidized and alkylated mRNAs accumulate in the absence of Xrn1.
From: Oxidation and alkylation stresses activate ribosome-quality control
a A representative HPLC-ECD chromatogram used to quantify the concentration of 8-oxoG in our samples. b Bar graphs showing the ratio of 8-oxoG to G in total and polyA-RNA in presence and absence of Xrn1. c Bar graphs showing that the ratio of 8-oxoG to G in total and polyA-RNA significantly increases when 4-NQO is added to growing yeast cells. d Bar graphs demonstrating the ratio of the indicated alkylation adducts relative to their respective unmodified nucleoside in polyA-RNA in the presence and absence of Xrn1. e Same as d but in the absence and presence of MMS. In all cases, bars represent the average (±SD) of at least three independent biological replicates. Source data are provided as a Source Data file.
