Fig. 3: The addition of damaging agents leads to the accumulation of Hsp104-mCherry foci in the absence of Ltn1 and Hel2. | Nature Communications

Fig. 3: The addition of damaging agents leads to the accumulation of Hsp104-mCherry foci in the absence of Ltn1 and Hel2.

From: Oxidation and alkylation stresses activate ribosome-quality control

Fig. 3

a Representative images of the indicated cells in the absence and presence of MMS. Shown are brightfield, mCherry and DAPI signals. Scale bar is 10 μm. b Bar graph showing the quantification of cells with >1 focus in the presence of the specified drugs for WT and ltn1Δ cells. c Similar to b, using WT and hel2Δ cells. d Bar graph used to show the consequence of a cycloheximide pre-treatment on the effect of MMS on foci formation. In all cases, the average foci count of at least three biological repeats is plotted with the error bars representing the standard deviation around the mean. For each biological repeat, > 120 cells were counted. Source data are provided as a Source Data file.

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