Fig. 5: Agents that strictly compromise the integrity of DNA do not lead to ribosomal proteins ubiquitination.
From: Oxidation and alkylation stresses activate ribosome-quality control
a Western blot analysis of ribosomal protein ubiquitination and uS3 in the presence of the indicated compounds. Phosphorylation of H2AX was used to assess the activation of the DNA-damage response. b Western blot analysis of ribosomal proteins ubiquitination as a function of increasing cisplatin concentrations. c Western blot analysis of ribosomal protein ubiquitination and H2AX phosphorylation as a function of time after the addition 4-NQO. Note ribosomal protein ubiquitination is observed only after 5 min of incubation and saturates at the ten-minute mark. In contrast, γ-H2AX signal appears not to near saturation after 45 min of incubation. Source data are provided as a Source Data file.
