Fig. 6: Mutations in NGD and RQC components render cells sensitive to 4-NQO and MMS addition.
From: Oxidation and alkylation stresses activate ribosome-quality control
a Top is a plot of OD600nm over time of the indicated strains after a mock treatment or a 30-minute challenge with 4-NQO (1 μg/mL) or MMS (0.1% MMS). Bottom is a plot of the first derivative of the data on top to calculate the instantaneous growth rate and the corresponding lag time needed to reach the maximum rate. Data were collected in technical duplicates from three biological replicates. b A bar graph showing the determined lag time from a. The mean of three biological replicates is plotted and the error bars represent the standard deviation. p value from two-way ANOVA test is indicated. Source data are provided as a Source Data file. c Images of spot assays performed using the indicated strains, after mock treatment or a 30-minute challenge using 4-NQO (1 μg/mL) or MMS (0.1% MMS).
