Fig. 6
From: ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Deeper ProTargetMiner dataset with 8 contrasting compounds is successful in target/MOA deconvolution. a OPLS-DA enabled deconvolution of multiple kinases as targets for dasatinib in three cell lines (drug targets shown in red circles). b deconvolution of DPP7 and DPP3 in MCF-7 and DPP3 in RKO cells. In A549 cells, these targets were not among the top 100 proteins. c identification of multiple targets for dasatinib in the merged dataset from three cell lines. d merging all cell lines shows that DPP3 is a common bortezomib target. Moreover, protein ubiquitination was the top GO term for 30 up-regulated proteins for bortezomib (11/30 proteins, p < 2E-06) in the merged dataset. e the specific down-regulation of DPP3 in response to bortezomib in MCF-7 cells. Data are represented as mean ± s.d. (n = 3 biologically independent experiments). Supplementary Data 4–7 were used for making panels a–e. Source data are provided as a Source Data file.
