Fig. 2: BarH-1 is expressed in white but not orange regions of the wing in C. crocea.
From: A transposable element insertion is associated with an alternative life history strategy

DAPI (nuclei, left, blue) and BarH-1 antibody (right, red) staining of pupal wings. Large nuclei are in scale building cells, small nuclei are in epithelial cells. The right part of the panel shows the approximate location of the stained area and the scales in this region in an adult female wing. Scale bars are 40 μM. a Staining of the forewing of an Alba female from black and b white regions. BarH-1 is expressed in black (melanic) as well as white Alba scale building cells. c Antibody staining of the forewing of an orange female in orange region. BarH-1 is not expressed in the orange scale building cells. d Antibody staining of the hindwing of an orange female. BarH-1 is heterogeneously expressed in the scale building cells within this region. This staining pattern presumably corresponds to the variation in scale color, with melanic (black) scale building cells expressing BarH-1 but orange lacking expression.