Fig. 1: Overview of SSiNGLe-SMS and SSiNGLe-ILM.

DNA with 3′-OH SSB (blue circle) is fragmented in situ with MNase that leaves 3′-P termini (red circles) that cannot be tagged with the polyA using TdT. After tailing, the native DNA is either sequenced directly on oligo(dT) flow cells (SSiNGLe-SMS) or put through the additional depicted steps for SSiNGLe-ILM.