Fig. 9: Butyrate downregulates AICDA and PRDM1 expression while upregulating AICDA- and PRDM1-targeting miRNAs in human B cells. | Nature Communications

Fig. 9: Butyrate downregulates AICDA and PRDM1 expression while upregulating AICDA- and PRDM1-targeting miRNAs in human B cells.

From: B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids

Fig. 9

Purified human B cells were stimulated with CD154 plus IL-4 and IL-21 in the presence of nil or butyrate (500 μM) for 60 h before preparation of RNA for mRNA-Seq and miRNA-Seq. a Profile of RNA-Seq reads at the AICDA and PRDM1 loci. b Scatter plots of miRNA expression as defined by miRNA-Seq (RPKs) in B cells treated with butyrate versus that in B cells treated with nil. c Bar graphs depict the fold changes in the average miRNA expression (RPMs) comparing B cells treated with nil or butyrate. d B-cell expression of AICDA- or PRDM1-targeting miRNAs, as analyzed by miRNA-Seq.

Back to article page