Fig. 5: Colocalization of compartment and TAD borders in growing cells.

a Numbers of TAD borders that were specific to growing or OIS cells. TAD borders were predicted from the growing (Bio1 and Bio2) and OIS (Bio1, Bio2, and Bio3) data. TAD borders consistently present in growing cell data but not in OIS cell data were classified as G-specific borders; TAD borders conserved in at least two OIS replicates but not in the growing data were categorized as OIS-specific borders. Remaining TAD borders detected in at least two datasets were classified as non-specific borders. b An example showing the colocalization of a compartmental border that was only present in growing cells (i.e., G-specific compartment border) and a TAD border that was only present in growing cells (i.e., G-specific TAD border). Contact maps in growing and OIS cells are shown for the same 4 Mb region in chromosome 3 (top); contact maps are depicted as in Fig. 2a. TAD borders (narrow red vertical lines) were predicted from border strength scores and are shown beneath the maps (Methods). PCA scores in growing and OIS cells are shown at the bottom. c Frequency of overlap between G-specific TAD borders and G-specific compartment borders, as exemplified in panel b. Overlap between different types of TAD borders and G-specific compartment borders was counted. Positions of TAD borders were allowed to shift by ± 1 bin (40 kb) to estimate the overlap with G-specific compartment borders. P-values were calculated by two-sided Student’s t-test, using biologically independent samples (n = 3, error bars represent the SD).