Fig. 5 | Nature Communications

Fig. 5

From: Chromosome-associated RNA–protein complexes promote pairing of homologous chromosomes during meiosis in Schizosaccharomyces pombe

Fig. 5

Liquid-phase separation of Smp proteins and its impact on homologous pairing. a–c Selected projected time-lapse images of live cells at the horsetail stage for Seb1-mCherry (a), sme2 RNA-U1Atag/U1Ap-mCherry (b), and A55-lacO/LacI-GFP (c). The sme2 RNA-U1Atag was visualized by U1Ap-mCherry11. Three-dimensional images were captured every minute. Microfluidic yeast plates (CellASIC) were used for cell culture on the microscope stage. 1,6-Hexanediol (10%) was added or removed, as indicated by the arrows. The solution in the cell culture chamber was changed at <1 min. Scale bar, 5 μm. d Number (%) of cells with paired chromosomes at sme 2 (left), A55 (middle), and C24 (right) loci. 1,6-Hexanediol was added to WT and rec12 deleted (rec12−) cells at 5 min and removed at 8 min (treatment period is shaded in the graphs). Three-dimensional live cell images were captured every minute, and the percentage of paired cells observed at 5-min intervals is shown. Source data for Fig. 5 are provided as a Source Data file 5.

Back to article page