Fig. 4: Effect of compounds on TNF:TNFR1 stoichiometry.
From: Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimer
a Analytical size exclusion chromatography (AnSEC) of human (h) TNF alone (blue trace), hTNF + UCB-5307 (red trace), hTNF + 3.2-fold excess hTNFR1 (green trace) and hTNF + 3.2-fold excess hTNFR1 + 690 µM UCB-5307 (brown trace). Expected migration position of hTNF with two and three receptors bound are indicated by arrows. Standard trace shown in Supplementary Fig. 5. b AnSEC comparing effect of 690 µM UCB-5307 on preformed hTNF/hTNFR1 complex (3.2-fold excess hTNFR1) (red trace) vs. hTNF preloaded with UCB-5307 followed by addition of 3.2-fold excess hTNFR1 (blue trace) as in experiment (a). Source data are provided as a Source Data file.
