Fig. 3: Functionally diverse latent and intrinsic AMP resistomes.

a Heatmap shows the corrected Jaccard similarity indices calculated for resistance-enhancing genes (blue) and sensitivity-enhancing genes (red) between AMP pairs based on the overexpression screen (see Methods for calculation of corrected Jaccard indices, n = 210, that is, the number of AMP pairs). The darker the color the higher the overlap of gene sets between AMP pairs. Source data are provided as a Source Data file. b The overlaps in the latent resistomes (genes enhancing resistance upon overexpression) between AMP pairs belonging to different chemical-genetic clusters. Significant difference: **P = 0.0045 from two-tailed unpaired t-test, n = 54 and n = 51 for between C1, C2, and C3, C4, and others, respectively. Boxplots show the median (center horizontal line), the first and third quartiles (bottom and top of box, respectively), with whiskers showing either the maximum (minimum) value or 1.5 times the interquartile range of the data. c Heatmap shows the corrected Jaccard similarity indices calculated for resistant (blue) and sensitive (red) chemical-genetic interactions with partially depleted essential genes (see Methods). Source data are provided as Supplementary Data 5. d Schematic figure showing sets of essential genes that simultaneously enhance AMP resistance when overexpressed and sensitivity when depleted. Color code is explained in the figure.