Table 1 Construction and characterization of the iMAGIC plasmid library.

From: Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

 

LibA

LibI

LibD

CRISPR protein

dLbCas12a-VP

dSpCas9-RD1152

SaCas9

Length of gRNAa

20 + 23 bp

20 + 82 bp

121 + 127 bp

No. of guides

37,817

37,870

24,806

Fold coverageb

~133×

~106×

~121×

Mapping ratio

~87.7%

~86.8%

~72.6%

gRNA coverage

~99.9%

100%

~88.9%

Gene coveragec

100%

100%

~98.3%

  1. aThe length of guide (underlined) and structural sequences
  2. bCalculated as estimated library size/number of guide sequences
  3. cAt least one guide for each gene
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