Fig. 2: Single-cell transcriptomics reveals different gene expression pattern in PAX7pos, PAX7neg, and PAX7null myogenic cells.
From: Human muscle-derived CLEC14A-positive cells regenerate muscle independent of PAX7
a–c Two-dimensional tSNE representation of global gene expression relationships among individual myogenic cells from a PAX7-deficient donor (PAX7null) and from donor A (PAX7neg-A, PAX7pos-A). Each dot represents a cell. a tSNE plot colored by cell cluster. b tSNE plot colored by cell population. c Normalized gene expression levels of selected marker genes (red: high, gray: low). d Violin plots indicating gene expression levels and distributions of selected genes within cell clusters corresponding to panel a. Clusters 0, 5, and 7 correspond to proliferating cells (MKI67). PAX7null cells express strongly elevated levels of CLDN11, FHL1, COL6A3, and CLEC14A in clusters 0 and 1, and TFPI2 in clusters 0, 1, and 2.
