Fig. 4: Stable expression of STAND proteins in the SNc.
From: An ultra-stable cytoplasmic antibody engineered for in vivo applications
a–l Immunohistochemical analysis of the SNc 33 days after injection with AAV9/3-STAND-A36 (a–c, g–i) or AAV9/3-STAND-M4 (d–f, j–l) a, d, g, j Anti-Flag antibody to detect scFv protein; b, e, h, k anti-tyrosine hydroxylase [TH] antibody used to detect dopaminergic neurons; c, f, i, l merged image of a and b, d and e, g and h, or j and k. g–l Magnified images of the SNc region in a–c or d–f. STAND proteins were stably expressed in the cytoplasm of dopaminergic neurons (arrows). Scale bars, 200 μm c, f and 50 μm i, l.
