Fig. 5: Stable expression of STAND proteins in a broad region of the SNc.
From: An ultra-stable cytoplasmic antibody engineered for in vivo applications
Brain sections (AP coordinates relative to bregma in mm: −2.92, −3.11, −3.3, −3.49, and −3.68) 33 days after injection with AAV9/3-STAND-A36 (a–e) or AAV9/3-STAND-M4 (f–j). STAND proteins (green) were expressed in tyrosine-hydroxylase (TH)-positive (magenta) dopaminergic neurons (white, in merged images). k Immunoblot analysis of total homogenates of the striatum and SNc from the left (L) and right (R) hemispheres of the mouse brain 33 days after injection with AAV-vectors; anti-Flag (STAND proteins), anti-TH, anti-Syt I, and anti-tubulin antibodies. Scale bar, 200 μm.
