Fig. 3: Formation of toroidal hexameric–dodecameric assemblies in solution.
From: Engineering protein assemblies with allosteric control via monomer fold-switching
a Size-exclusion chromatography of CI2eng. The chromatogram shows one peak corresponding to the monomer (M) and a broader peak for the mixture of hexamer (H) and dodecamer (H2). b Analytical ultracentrifugation of CI2eng resolving the three species: monomer (M), hexamer (H), and dodecamer (H2). c (Left) cryo-image of CI2eng in solution. Arrows point to end-on views and arrowheads to tilted views of the particles. (Right) gallery of representative 2D classes classified with Relion68. Doughnut shapes represent end-on views and the other shapes various side views of the CI2eng assembly. d 3D reconstruction at sub-nm resolution of the CI2eng assembly formed in solution.
