Fig. 5: NOXA is induced by STING-dependent extrinsic paclitaxel-induced apoptotic priming signals. | Nature Communications

Fig. 5: NOXA is induced by STING-dependent extrinsic paclitaxel-induced apoptotic priming signals.

From: STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment

Fig. 5

a, b Immunoblot (a) and PMAIP1 qPCR (b) analysis in MDA-MB-231 recipient cells 48 h incubated with #1 or #2 consecutively produced CM as described in the protocol depicted in the figure. c Immunoblot and PMAIP1 qPCR analysis in PDX treated or not by paclitaxel after indicated times. d After a 24 h paclitaxel-treatment or not, (donor) breast cancer cells were washed out to produce 48 h-CM that were applied to control or NOXA−/− untreated (recipient) corresponding cancer cells for 48 h in presence or not of the BH3 mimetic WEHI-539. Apoptotic index in recipient breast cancer cells was assessed using Annexin-V staining. e NOXA immunoblot (left panel) and PMAIP1 qPCR (right panel) analysis in MDA-MB-468 recipient cells incubated for 48 h with 48 h-CM from control, STING−/− or TNFα−/− donor cells treated or not with paclitaxel (upper panel) and in control or IFNAR1−/− MDA-MB-468 recipient cells with 48 h-CM from control donor cells treated or not with paclitaxel (lower panel). f Immunoblot and PMAIP1 qPCR analysis in MDA-MB-468 cells treated for 48 h or not with recombinant TNFα and/or IFNα. g Apoptotic effect of recombinant IFNα and/or TNFα on control or NOXA−/− MDA-MB-468 cells treated or not with WEHI-539 during 48 h. Data were collected from at least n = 3 independent experiments. Error bars indicate mean +/− SEM; Two-sided paired t-test. The symbols correspond to a p-value inferior to *0.05 and **0.01.

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