Fig. 4: Inhibition of vSUB terminals targeting the VS impairs spatial memory consolidation. | Nature Communications

Fig. 4: Inhibition of vSUB terminals targeting the VS impairs spatial memory consolidation.

From: Offline ventral subiculum-ventral striatum serial communication is required for spatial memory consolidation

Fig. 4

a Schematic of the ex vivo electrophysiology DREADDs experiment. b Representative traces from VS MSNs showing sEPSCs in control conditions (black) and under CNO (orange) c Scatter plot graph summarizing sEPSC frequency in control conditions (black) and under CNO (orange) (n = 13 cells/6 mice; t12 = 2.8, p = 0.015; paired t-test). d CNO did not change the sEPSCs amplitude (p = 0.38; paired t-test). e Schematic of the behavioral DREADDs experiment. f Schematic of AAV expressing Venus and the HA-tagged hM4Di under the h-Synapsyn (Syn) promoter. Representative coronal sections from HPC and VS. Scale bars: 1 mm. g Mice infected in the vSUB with the AAV and injected post-training with CNO (10 mM) in the VS showed impaired performance on the probe test. Histograms represent the mean of distance traveled in each quadrant for vehicle (n = 7) and CNO (n = 13) injected groups (repeated measures ANOVA of quadrant preference F3,54 = 22.976, p = 0.0001; treatment F1,18 = 0.085, p = 0.7735; quadrant preference × treatment F3,54 = 8.019, p = 0.0002). The insert shows first bearing of mice in the two groups (t17 = 3.450; p = 0.0031; unpaired t test). Bottom panels: representative path from vehicle and CNO animals. h Mice bilaterally infected in the vSUB and injected 120 min post-training with CNO (10 mM) in the VS showed no impairment in the performance. Histograms represent the mean of distance traveled in each quadrant for vehicle (n = 11) and CNO (n = 9) injected groups (repeated measures ANOVA of quadrant preference F3,54 = 24.6954, p < 0.0001; treatment F1,18 = 0.4137, p = 0.3721; quadrant preference × treatment F3,54 = 0.8742, p = 0.46022). The insert shows first bearing of mice in the two groups (t18 = 0.5077; p = 0.6178; unpaired t-test). Bottom panels: representative path from vehicle and CNO animals. Histograms represent mean ± SEM. #p < 0.05 target vs target (between groups); *p < 0.05 target quadrant vs right, opposite, left (within group, Tukey). Bottom panels: representative probe-trial. MWM images modified from ref. 9; Image credit for brains schematics: Allen Institute.

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