Fig. 4: Unbiased whole-genome amplification of low-quantity genomic DNA.
From: Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
a, b gDNA was prepared by two different methods for transposon sequencing. For the WGA sample, 10 ng of gDNA was amplified first with DNA polymerase phi29 before MmeI digestion and adapter ligation. For the standard sample, 1 μg of gDNA was digested with MmeI, followed by adapter ligation. There is a strong correlation between fitness values obtained from WGA preparation compared with standard Tn-Seq library preparation a, and WGA preparation is highly reproducible b.
