Fig. 7: Treatment of a DSS-induced mouse model of colitis with LCB. | Nature Communications

Fig. 7: Treatment of a DSS-induced mouse model of colitis with LCB.

From: Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment

Fig. 7

a Experimental design for treatment. Mice were fed with 3% DSS for 7 days to develop colitis, and then administrated with 1 × 108 CFU of LCB daily for a further 5 days. Uncoated EcN and PBS were used as controls. The intestinal tract of treated mice was harvested for blinded histopathological analysis 5 days post-treatment. b, c The (b) weight and (c) length of the intestine tract 5 days post-treatment. d Histopathology scores of ceca after treatment with PBS, uncoated EcN and LCB, respectively. e, f Representative images of (e) H&E and (f) MPO staining of ceca. g Mean MPO positive cells counted in ceca. Black arrow: shedding of mucosal epithelium cells; Yellow arrow: disappearance and replacement of intestinal glands in lamina propria; Red: Lymphocyte infiltration. Error bars represent standard deviation (n = 5). Significance was assessed using Student’s t-test, giving p values, *p < 0.05, **p < 0.01, ***p < 0.005. Scale bar: 50 μm.

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