Fig. 1: RLD genes regulate GSA in LRs and embryo development.
From: Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
a Schematic diagram of RLD. b, c Twelve-day-old seedlings of Col (b) and rld1-2 rld4-1 double mutant (c). Arrows indicate the growth orientation of lateral roots. d Lateral root tip angle is represented by boxplots for 12-day-old seedlings of Col, rld1-2, rld4-1, and rld1-2 rld4-1 toward the direction of gravity. Median and quartile values are provided by the central line and box boundaries. Whiskers show min to max values. n, sample number of three biologically independent experiments. Asterisks indicate significant differences by the Tukey–Kramer method (P < 0.05). e Comparison of root curvature of 5-day-old seedlings of each line at 12 h after a 90° reorientation. n, sample number of three biologically independent experiments. Asterisks show significant differences by the Tukey–Kramer method (P < 0.05). f, g Embryos of Col (f) and rld1-2 rld2-2 rld3-2 rld4-1 (g). h–j Seven-day-old seedlings of Col (h) and rld1-2 rld2-2 rld3-2 rld4-1 (i, j). k–m Seven-day-old seedlings of Col (k) and partial complemented rld1-2 rld2-2 rld3-2 rld4-1 seedlings by expressing RLD1-mCherry under control of the ATHB8 promoter (l, m). n RLD1-mCherry expression in the primary root of 5-day-old rld1-2 rld2-2 rld3-2 rld4-1 seedling harboring ATHB8p:RLD1-mCherry. A double-head arrow indicates RLD-mCherry expression domain in the vasculature. Arrow marked with “g” represents the direction of gravity. Scale bars, 1 cm (b, c), 100 μm (f, g), 5 mm (h–j), 2 cm (k–m), and 50 μm (n). Source data for d, e are provided as a Source Data file.
