Fig. 3: LZY recruits RLD to the plasma membrane via the CCL-BRX interaction.
From: Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
a, b Co-expression of RLD1-GFP with mCherry (a) and LZY3-mCherry (b) in Arabidopsis protoplast cells. Arrows indicate plasma membrane-localized signals. c Localization of RLD1-GFP (left) and LZY2-mCherry (middle) in primary root of 8-day-old seedling harboring 35S:RLD1-GFP and G10-90p:XVE»LZY2-mCherry, transferred to MS medium plate containing 1 μM estradiol at 7 days old. Merged image of RLD1-GFP and LZY2-mCherry (right). Arrowheads indicate LZY2-mCherry-expressing cells, where RLD1-GFP was co-localized with LZY2-mCherry in the plasma membrane. d, e Co-expression of RLD1-GFP and CCL-deleted LZY3-mCherry (LZY3ΔCCL) (d), and of the BRX domain-deleted RLD1-GFP (RLD1ΔBRX) and LZY3-mCherry (e) in Arabidopsis protoplast cells. f, g Co-expression of the BRX domain (BRXd)-mCherry with GFP (f) and LZY3-GFP (g) in Arabidopsis protoplast cells. h, i Co-expression of RLD1-GFP with mCherry-LTI6b (h) and CCL-mCherry-LTI6b (i) in Arabidopsis protoplast cells. Scale bars, 10 μm (a, b, d–i) and 20 μm (c). j, k Interaction between LZY3 and truncated forms of RLD1 and RLD2 (j), and between C terminus of RLDs and truncated forms of LZY3 (k) in the Y2H system. Interaction was indicated by growth on selection medium lacking leucine, tryptophan, histidine, and adenine.
