Fig. 4: The structure of the CCL-BRX complex.
From: Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
a Binding of the RLD2 BRX domain to the LZY3 CCL peptide. The obtained ITC profile showed formation of a 1:1 complex with a small KD value (9.7 ± 2.2 nM). ΔH, TΔS, and N are −11.3 ± 0.09 kcal/mol, −1.68 kcal/mol, and 0.73 ± 0.002, respectively. b Topology diagram of the RLD2 BRX domain bound to LZY3 CCL. c Ribbon representation of the crystal structure of the BRX-CCL complex. Color codes are as in b. The RLD2 BRX domain comprises a three-stranded antiparallel β-sheet (blue) and two α-helices (green). The LZY3 CCL peptide adopts a β-hairpin structure (pink). Dashed lines represent inter-molecular hydrogen bonds. The colors of corresponding positions in the CCL-BRX complex at which mutations were introduced in RLD1-GFP and LZY3-mCherry are reversed.
