Fig. 6: Asymmetric localization of LZY3-mCherry, RLD1-GFP, and PIN3-GFP to the direction of gravity.
From: Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
a–d The localization of LZY3-mCherry in the LR tips of 8-day-old lzy1 lzy2 lzy3 seedlings harboring LZY3p:LZY3-mCherry at the stage 2 before rotation (a) and at 5 min (b), 30 min (c), and 60 min (d) after 180° rotation. White and yellow arrowheads indicate polarized LZY3-mCherry localization and the direction of gravity estimated from the growth orientation of LR tips, respectively. Double-headed arrows show the apical-basal axis of the plant body. Asterisks indicate central columella cells. e, f Comparison of asymmetric LZY3-mCherry localization in columella cells (e) and central columella cells (f) in LR tips at stage 2 before and after 180° rotation. Median and quartile values are provided by the central line and box boundaries. Whiskers show min to max values. g–j The position of amyloplasts in columella cells of LRs of 8-day-old lzy1 lzy2 lzy3 seedlings harboring LZY3p:LZY3-mCherry before and after 180° rotation. Brown arrowhead indicates a biased distribution of amyloplasts in columella cells. k–n The localization of RLD1-GFP in LR tips of 8-day-old seedlings harboring RLD1p:RLD1-GFP before and after 180° rotation. o–q The localization of PIN3-GFP in the LR tips of 8-day-old seedlings harboring PIN3p:PIN3-GFP before and after 180° rotation. Arrowheads indicate lateral columella cells, adjacent to central columella cells (red, apical flank; white, basal flank). r Measurement of PIN3-GFP fluorescence intensity at lateral plasma membrane domains of columella cells adjacent to central columella cells at the basal flanks (white) compared with those at the apical flanks (red) in LR tips of 8-day-old seedlings harboring PIN3p:PIN3-GFP. s Comparison of asymmetric localization of PIN3-GFP in the LR tips of 8-day-old seedlings harboring PIN3p:PIN3-GFP before and after 180° rotation. n, sample number of three biologically independent experiments (e, f, s). Different letters in e, f, s indicate statistical differences (Tukey–Kramer, P < 0.05). Source data for e, f, and s are provided as a Source Data file. Scale bars, 20 μm.
