Fig. 5: Suppression of histone modifications on Il17a promoter by DMF is GSH-dependent. | Nature Communications

Fig. 5: Suppression of histone modifications on Il17a promoter by DMF is GSH-dependent.

From: IL-17+ CD8+ T cell suppression by dimethyl fumarate associates with clinical response in multiple sclerosis

Fig. 5

a ChIP assays for H4Ac and H3K27Ac at the Il17a promoter, Il17 enhancer-5 in murine Tc17 cells differentiated for 72 h ± DMF or DMF + GSH. b ChIP assays for H4Ac and H3K27Ac at the Il10 promoter in murine Tc17 cells differentiated for 72 h ± DMF or DMF + GSH. c ChIP assays for H3K4me3 at the Il17 promoter, Il17 enhancer-5 in murine Tc17 cells differentiated for 72 h ± DMF or DMF + GSH. d ChIP assays for H3K4me3 at the Rpl32 promoter in murine Tc17 cells differentiated for 72 h ± DMF or DMF + GSH. Bars show mean ± s.d. of fold change normalized to DMF treatment, which was arbitrarily set to 1. Data from three a–d combined experiments; individual values are plotted. In a–d, *p < 0.05, **p < 0.01, ***p < 0.001, evaluated by one-way ANOVA followed by Tukey’s HSD multiple comparison test.

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