Fig. 3: Conditional control of Cas13a cleavage.
From: Conditional control of RNA-guided nucleic acid cleavage and gene editing
Reactions were carried out as described in the Experimental section. All samples were tested in three biological replicates. Image of representative data is shown here. The fluorescence of the sample versus time was shown. a, c, e The influence of chemical masking on Cas13a cleavage. The crRNA13a was masked with NAI-N3 (200 mM) for different durations. b, d, f The influence of DPBM on Cas13a cleavage. The CRISPR/Cas13a system with masked crRNA13a (200 mM NAI-N3, 2.0 h) was treated with various concentrations of DPBM. Source data is available in the Source Data file.
