Fig. 4: Chemical masking protects gRNA from RNase degradation.
From: Conditional control of RNA-guided nucleic acid cleavage and gene editing
Reactions were carried out as described in the Experimental section. All samples were tested in three biological replicates. Lanes 1, 5, 9, 13: no RNase I control; lanes 2, 6, 10, 14: RNase I cleavage for 2.5 min; lanes 3, 7, 11, 15: RNase I cleavage for 10 min; lanes 4, 8, 12, 16: RNase I cleavage for 30 min. Source data is available in the Source Data file.
