Fig. 5: Interactions of crRNA13a with both Cas13a and target RNA.
From: Conditional control of RNA-guided nucleic acid cleavage and gene editing
a Representative melting profiles of the crRNA/target RNA with different treatments were recorded in 10 mM Tris-HCl buffer (pH 7.0, 100 mM NaCl). Melting temperature of the NAI-N3-treated sample was statistically significantly different from that of the no treatment control (P < 0.05). b The dCas13a was incubated with 5′-Cy3-labeled crRNA probe (crRNA13a-Cy3 in Supplementary Table 1) with different treatments, and the samples were separated on native 6% PAGE gels. Lanes 1, 3, 5, 7, 9: no protein control; lanes 2, 4, 6, 8, 10: crRNA13a was incubated with a 5.0 molar excess of protein. Source data is available in the Source Data file.
