Fig. 6: Gene editing in live cells.
From: Conditional control of RNA-guided nucleic acid cleavage and gene editing
The T7E1 nuclease assay was performed 24 h post-transfection using Cas9-expressing HeLa-OC cells transfected with gRNA-HBEGF with different treatments. a Uncleaved HBEGF DNA (621 bp) cut to shorter cleavage fragments (311 bp and 310 bp) were indicated. Lane 1: target control; lane 2: no gRNA-HBEGF control; lane 3: original gRNA-HBEGF; lane 4: original gRNA-HBEGF, 300 μM DPBM; lanes 5–9: masked gRNA-HBEGF (200 mM, 1 h), different concentrations of DPBM; lane 10: DNA markers. The “Lanes and Bands” tool in Image Lab software version 5.1 (Bio-Rad) was used for image acquisition and differential densitometric analysis of the associated bands from the gels. Although long exposure times were necessary to visualize faint bands, the calculations of indel formation were not influenced by the extent of exposure. b The Cyan color represents the original gRNA-HBEGF group, while the Blue color represents the masked gRNA-HBEGF group. Error bars: ±SEM. Source data are provided as a Source Data file.
