Fig. 6: Illustration of the SRS system and SRS images of fixed HeLa cells. | Nature Communications

Fig. 6: Illustration of the SRS system and SRS images of fixed HeLa cells.

From: Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging

Fig. 6

a Schematic illustration of the instrumental setup of the SRS imaging system. EOM: electro-optic modulator; DM: dichroic mirror; GM: galvanometer; F: optical filters; PD: photodiode. b Stimulated Raman transition process for vibrational imaging of HeLa cells stained by PDDA-based tags. c SRS images (2120 cm−1) of fixed HeLa cells treated with 50 μM of P2, P3, and P4, respectively. Scale bar: 10 μm. d SRS spectrum of the HeLa cell sample stained with P2 in the red box region in (c), the spontaneous Raman spectrum of P2 in aqueous solution shown as a reference.

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