Fig. 7: Accumulation of 8-oxoG in the initial stages of iPS generation.
From: Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency
a 8-oxo-dG staining at day 3 post-induction using Yamanaka factors (mouse Dox-inducible system). The MEFs used were derived from a chimeric mouse generated with a GFP-positive iPSC line containing the Dox-inducible transgene constructs encoding the four reprogramming factors (see Supplementary Fig. 9a). Scale bar, 100 µm. Error bars show the SD of the mean (n = 10 fields randomly selected of two independent dishes). For statistical analysis, the Mann–Whitney U-test was employed. b 8-oxo-dG staining at day 3 post-infection (human retroviral system). Human fibroblasts (BJ cells) were infected with OSKLN cloned into a retrovirus all-in vector. The method used for quantitative imaging is shown in Supplementary Fig. 21b. Scale bar, 100 µm. Error bars indicate the SD of the mean (n = 10 fields randomly selected of two independent dishes). For statistical analysis, the Mann–Whitney U-test was employed. Source data are provided as a Source Data file.
