Fig. 3: Microglia activation and peripheral macrophage infiltration persist at 28 days post SNI.

a–c Immunostaining of Iba1+ microglia in the dorsal horn (a), CSF1R-GFP+ macrophages (green) in the DRG (b), and at the nerve injury site (c), 28 days after SNI (POD28). NF200 (blue) labels neuronal cell bodies (b) and peripheral nerve myelinated axons (c). Dashed line in c denotes nerve ligature site. Scale bar: 50 µm in a; 15 µm in b, c.