Fig. 1: RNF208 is significantly underexpressed in highly metastatic breast cancer cells. | Nature Communications

Fig. 1: RNF208 is significantly underexpressed in highly metastatic breast cancer cells.

From: RNF208, an estrogen-inducible E3 ligase, targets soluble Vimentin to suppress metastasis in triple-negative breast cancers

Fig. 1

a Volcano plot of RNF208 expression in luminal subtype (MCF-7, T47D, and ZR-75B) and TNBC (MDA-MB-231 and Hs578T) cells using RNA sequencing (GSE100878). b RT-PCR and immunoblot analysis of RNF208 expression in breast cancer cells. c Scatter plots of RNF208 expression in 52 breast cancer cells from public microarray datasets (GSE41313) (Luminal N = 29 and TNBC N = 23 cells). d, e Heat map and box plots showing the expression levels of RNF208, ESR1, and ERBB2 across the breast cancer subtypes using Genomic Data Commons (GDC). (Luminal A N = 335, Luminal B N = 88, HER2 N = 23 and TNBC N = 55 patients). f Comparison of RNF208 expression in breast cancer subtypes using published microarray datasets (GSE2034) (Luminal N = 154 and TNBC N = 55 patients). g Representative immunohistochemical (IHC) staining of RNF208 protein expression classified in luminal and basal breast cancer patient tissues. Original magnification ×100. Scale bar, 50 μm. h Real-time quantitative RT-PCR of RNF208 expression in luminal subtype tissues (N = 6) and TNBC tissues (N = 6); 18S rRNA was used as an internal control. i Kaplan−Meier analysis showing relapse-free survival depending on RNF208 expression levels from public meta-analysis data (N = 3951). P values were calculated using a log-rank test. All P values were calculated by unpaired two-tailed Student’s t tests (c, e, f, and h). These data represent the mean ± S.D. Source data for (c, f, h) is available in Source Data file. Unprocessed original scans of blots and gels in (b) are shown in Supplementary Fig. 13.

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