Fig. 4: ATP promotes activator dissociation by sequestering magnesium ions.
From: Polyanions provide selective control of APC/C interactions with the activator subunit
a The effect of the indicated nucleotides on Cdh1 dissociation was tested using phenol–chloroform extracted RNA species from the hydroxyapatite flow-through fraction (Fig. 2b). Reactions were supplemented with 2.5 mM MgCl2 and 3 mM of the indicated nucleotides. Uncropped autoradiograph and source data are provided in the Source Data file. b 3 mM ATP, inorganic pyrophosphate (PPi), or tri-phosphate (PPPi) were tested in a Cdh1-dissociation assay with reaction buffer or 1 μM 75mer ssDNA oligonucleotide. Reactions contain 2.5 mM MgCl2. Uncropped autoradiograph and source data are provided in the Source Data file. c–f A 75mer ssDNA oligonucleotide c, d or polyphosphate e, f was tested in the Cdh1-dissociation assay in the presence c, e or absence d, f of 2.5 mM MgCl2. Reactions were supplemented with 0 mM (black) or 3 mM ATP (red). Data indicate means (±SEM) from two independent experiments. Uncropped autoradiographs and source data are provided in the Source Data file. g The inhibitory effect of various MgCl2 concentrations was analyzed in Cdh1-dissociation reactions. Reactions were performed using 1 μM 75mer ssDNA with (red) or without (black) 3 mM ATP. Data indicate means (±SEM) from two independent experiments. Uncropped autoradiographs and source data are provided in the Source Data file.
