Fig. 1: Clonal diversity of CD8⁺ CAR-T cells decreases after infusion.

a CD8⁺/EGFRt⁺ CAR-T cell counts in the blood of patients who received CAR-T cells manufactured from CD4⁺ and CD8⁺ T_CM cells (n = 10 patients). Data represents the mean ± SEM. ALL acute lymphoblastic leukemia, NHL non-Hodgkin lymphoma. b The Morisita index was calculated for pairwise comparisons of the CD8⁺ CAR-T cell repertoire between all samples. Left, Morisita overlap index between the IP and the indicated times after adoptive transfer. Each line represents data from an individual patient. Right, heatmap of the Morisita index. The Morisita index was lower at late compared to early times after infusion (paired Mann–Whitney test, p < 0.05). c Decreased clonal diversity in CD8⁺ CAR-T cells after infusion. The Shannon entropy indices of the CD8⁺/EGFRt⁺ (CAR-T cell, left) and CD8⁺/EGFRt⁻ (non-CAR-expressing T cell, right) TCRB repertoires are shown. Statistical differences between samples were evaluated with paired t-tests. *p < 0.05, **p < 0.005, ***p < 0.001. d The relative frequencies of the top ten ranked clonotypes in the total CD8⁺ CAR-T cell TCRB repertoire in the IP and at the early and late time points after infusion are presented in pie charts. The top ten clonotypes are ranked by the number of copies of the TCRB sequence in each sample; the same color might not represent the same identical clonotype at each time point. Source data underlying Fig. 1a, c, and d are provided as a Source Data file.