Fig. 5: Phylogenetic Tree of CD34+ progenitors and proportion of clones in MPN04.
From: Single-cell analysis based dissection of clonality in myelofibrosis
MPN04 showed a complete change in clonal architecture due to an acquired LOH of FGF1 V66M on chromosome 5q31 between the two investigated time points (before and after leukemic transformation). At first time point, a dominant clone harboring mutations in CALR, FGF1, SUZ12, and TRPM5 was present, from which a subclone acquired a del(5)(q23-q32), leading to wild-type FGF1. At the second time point, this subclone developed additional genetic abnormalities affecting CALR and TRPM5. Top panel: bar chart displaying the proportion of each observed subclone. Middle panel: genotype matrix for each subclone. Bottom panel: Evolutionary trees generated by analysis of the single-cell data. A single-phylogenetic tree was constructed and displayed as a vertically oriented rectangular cladogram. The root of the tree harbors a wild-type cell genotype. Branch lengths are indicated (proportional to the number of evolutionary changes inferred) and the internal nodes (the points at which branches diverge) represent the ancestral clade from which arise all genotypes at the leaves/ tips of the tree (descendant subclones). Source data are provided as a Source Data file.
