Fig. 2: Degradation of substrates with K48-linked polyubiquitin chains. | Nature Communications

Fig. 2: Degradation of substrates with K48-linked polyubiquitin chains.

From: The proteasome 19S cap and its ubiquitin receptors provide a versatile recognition platform for substrates

Fig. 2

Degradation of substrate proteins with K48-linked ubiquitin chains by the indicated proteasome mutants was followed under single-turnover conditions (5 nM substrate, 25 nM proteasome) in the presence of 1 mM ATP at 30 °C. The graphs show substrate fluorescence as a percentage of the initial fluorescence as a function of time in minutes. Proteasome types are described in Supplementary Table 1 (TM triple mutant proteasome). Each panel shows the degradation of particular substrates, and as follows: a Ub5(K48)-GFP-35; b Ub9(K48)-GFP-35; c Ub5(K48)-GFP-95, and d 95-GFP-Ub5(K48). Source data are provided as a Source Data file.

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