Fig. 7: Degradation of multi-ubiquitinated substrates.
Degradation of substrate proteins modified with two K48-linked ubiquitin chains or bearing two co-translated single ubiquitin domains by the indicated proteasome types was followed under single-turnover conditions (5 nM substrate, 25 nM proteasome) in the presence of 1 mM ATP at 30 °C. The graphs show substrate fluorescence as a percentage of the initial fluorescence as a function of time in minutes. Proteasome types are described in Supplementary Table 1 (TM triple mutant proteasome). Each panel shows the degradation of particular substrates, and as follows: a Ub3(K48)-35-Ub3(K48)-GFP-35 and b Ub-35-Ub-GFP-35. Source data are provided as a Source Data file.
