Fig. 2: Loss of adipose OGT promotes lipolysis in visceral fat.
From: O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity
a Tissue weight of eWAT from WT and OGT AKO mice in fed, 6 h fasted, 24 h fasted, and 6 h refed states (n = 3–5 WT, 3–4 KO). b Changes in eWAT tissue weight during fasting/refeeding (n = 3–5 WT, 3 KO). c Hematoxylin–eosin (H&E) staining of eWAT from 2-month-old WT and OGT AKO mice in fed and 24 h fasted states, scale bar is 20 μm. d, e Quantification of adipocyte size in eWAT shown in c (three experiments). f Dynamic changes in serum FFA level in WT and OGT AKO mice during fasting/refeeding (n = 4–8 WT, 4–6 KO). g Basal and stimulated (10 μM CL-316,243) lipolysis measured by glycerol released from explants of eWAT and iWAT and isolated brown adipocytes from WT and OGT AKO mice (n = 2–6/group, three experiments). Data are presented as mean ± s.e.m. Statistical analysis: Student’s t test, *p < 0.05, **p < 0.01, and ***p < 0.001. Source data are provided as a Source Data file.
