Fig. 6: CDX2GFP+ sorted organoids show conserved regional specificity.
From: Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells
a–c Sections of paraffin embedded organoids cultured in either IM + CK or CK + DCI stained for Chromogranin A (CHGA), Lysozyme (LYZ), Villin (VIL), Cdx2, and colonic mucin Muc2 (scale bar = 50 μm, representative of n = 5 organoids from n = 2 differentiations). d qRT-PCR for various genes of interest, from D54 HIOs cultured in either CK + DCI or IM + CK as compared with a primary control normalized to day 0 hiPSCs (2−ΔΔCT, technical triplicates normalized to GAPDH or ACTB (β-ACTIN), n = 3 differentiations, error bars represent the s.d., statistical significance where indicated determined by unpaired Student’s t-test, *p < 0.05).
