Fig. 2: RIPO(H3.3) expresses and processes the foreign H3.3K27M antigen. | Nature Communications

Fig. 2: RIPO(H3.3) expresses and processes the foreign H3.3K27M antigen.

From: Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity

Fig. 2

a Processing map for RIPO(H3.3); the H3.3K27M epitope is shown in red. b Top panel. Viral P1 and VP0 precursor polypeptides fused to the H3.3K27M epitope were detected. We observed an additional, weaker band in RIPO(H3.3)-infected cells that cannot be assigned to any of the predicted viral precursor polypeptides in size (*). Since the signal strength of this band over time recapitulated the pattern of H3.3K27M-P1, we assume that this band represents a degradation product of H3.3K27M-P1. Bottom Panel. eIF4G1 cleavage, viral P2/2BC/2C expression in HeLa cells infected with RIPO(H3.3). 12-O-Tetradecanoylphorbol-13-acetate (TPA) was used to stimulate viral translation64.

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