Fig. 3: mOVA2 activates DCs and mediates SIINFEKL epitope presentation to CD8 T cells. | Nature Communications

Fig. 3: mOVA2 activates DCs and mediates SIINFEKL epitope presentation to CD8 T cells.

From: Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity

Fig. 3

a, b Immunoblot and flow cytometry analysis of GMCSF- (a) and FLT3L- (b) BMDCs showing type I IFN-dominant activation and upregulation of costimulatory molecules upon infection with mOVA2. For co-stimulatory molecule expression, asterisks denote statistically significant differences from mock (0 h)-treated samples as determined by Two-way RM-ANOVA protected Dunnett’s post hoc test; (a) n = 4; (b) n = 3. Repeat immunoblot assays and gating strategy are shown in Supplementary Fig. 6a–c, respectively. c, d Multiplex ELISA showing cytokine profiles of infected GMCSF- (c) and FLT3L- (d) BMDCs. Asterisks denote statistically significant differences from mock (0 h)-treated samples determined by One-way ANOVA protected Dunnett’s post hoc test; n = 3. e GMCSF-BMDC/OT-I co-culture assays. Asterisks denote statistically significant differences determined by Two-way ANOVA protected Sidak’s multiple comparison test, n = 3. The assay was performed three times; representative results are shown. a–e Error bars denote SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = number of independent replicates.

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