Table 1 Dependence of structural characteristics of full-length Bvht (636 nucleotides, 206 kDa) on Mg2+ concentration.

From: Zinc-finger protein CNBP alters the 3-D structure of lncRNA Braveheart in solution

Mg2+ concentration

0 mM

6 mM

12 mM

Rg (Å)

98.1 ± 0.2

99.53 ± 0.2

84.8 ± 0.7

Dmax (Å)

300

287

260

χ2

1.3

1.28

1.18

NSD

1.27 ± 0.02

1.21 ± 0.04

1.24 ± 0.04

Resolution (Å)

13.9–18.6

13.6–35.0

14.2–37.2

  1. Rg was determined using the P(r) function that utilizes both lower and higher angle datasets for each sample. Dmax is the radius where the P(r) distribution approaches to zero. The χ2 value indicate the goodness of fit between the experimentally collected and model-derived SAXS data, whereas the NSD (normalized spatial discrepancy) describes an agreement between individual models for each dataset. The resolution of models was calculated by phenix.mtriage63. See Methods section for additional detail
Back to article page