Fig. 3: Liprin-α promotes the clustering of LAR.
From: Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity
a, b Cell imaging of over-expressed N-terminal GFP-tagged liprin-α1 (a) and C-terminal Flag-tagged LAR (b) in COS7 cells. c–g Cells co-transfected with GFP-tagged liprin-α1 wild-type or its variants and Flag-tagged LAR or its variants. Regions of interesting were highlighted by yellow boxes, enlarged four times, and aligned on the right of each merged image. “WQ” and “FE” indicate the W896Q mutant of liprin-α1 and the F1829E mutant of LAR, respectively. Scale bar, 10 μm. h Quantification of the LAR clustering levels as shown in (a–g) and in Fig. 4c. Error bars represent s.d. ~15 cells per experimental condition. The unpaired Student’s t test analysis was used to define a statistically significant difference (*p < 0.05; **p < 0.01; ***p < 0.001; ns not significant).
