Fig. 6: Overlap between ETS1/FLI1 and PU.1-binding sites.

a IGV genome browser tracks of the RASSF2 locus showing average PU.1 (blue), ETS1 (purple), and FLI1 (pink) ChIP-seq coverage in control (mutPU.1) and PU.1-expressing cells. ATAC-seq coverage of PU.1-transfected and control cells are depicted in blue below the ChIP-seq tracks. b De novo-derived motif enrichment across the indicated ChIP-seq peaks. c Correlation matrix heatmap for position weight matrices (PWM) of the motifs shown in b. d Venn–Euler diagram showing the overlap of ETS1 and FLI1 ChIP-seq peaks (using stringent and standard peak calling). e Distribution of PU.1, ETS1, and FLI1 ChIP-seq, as well as ATAC-seq signals before and after PU.1 expression plotted across the ATAC-seq-derived PU.1 peak clusters (top panel), as well as regions that lost accessibility after PU.1 induction (bottom panel) in CTV-1 cells, as introduced in Fig. 3. f Bar plots displaying the overlap of stringent ETS1 (left panel) and FLI1 (right panel) peaks in PU.1-transfected (blue/purple bars) and control CTV-1 cells not expressing PU.1 (gray bars) with PU.1 peaks across the PU.1 peak clusters introduced in Fig. 3. g Motif log odds score distribution of the consensus ETS class 1 motif is shown for FLI1-overlapping peaks across ATAC-seq-derived PU.1 peak clusters along with FLI1 specific (sp) peaks. The median of each distribution is depicted inside the bean with a conventional boxplot. b–g Source data are provided as a Source Data file.