Fig. 5: bNAbs and ART modulate adaptive immunity. | Nature Communications

Fig. 5: bNAbs and ART modulate adaptive immunity.

From: Single-dose bNAb cocktail or abbreviated ART post-exposure regimens achieve tight SHIV control without adaptive immunity

Fig. 5

a–d Plasma IgG binding titers to SF162 Env protein (gp140) were measured by ELISA. Group colors and individual animal symbols are consistent throughout the manuscript. The animal number is indicated for animals that had positive antibody responses to SF162 gp140. b, c In Groups 2B and 3, the period of bNAb decay (rather than endogenous antibody response) is indicated by arrows. e–h T cell responses to HIV-1 Env antigen, SIVmac239 Gag ORF 15-mer peptide pool, or SIVmac239 Vif ORF 15-mer peptide pool were measured by IFNγ ELISPOT. SF162 gp140 protein was used as the Env antigen for all samples except for peripheral blood mononuclear cells (PBMC) for Groups 3 and 4 (g, h), for which Clade B consensus Env ORF 15-mer peptide pools were used. T cell responses in PBMC, mesenteric lymph nodes (MesLN), and spleen were quantified at time of death. Concanavalin A (ConA) and/or Staphylococcal Enterotoxin B (SEB) were used as positive stimulation controls. For each group, horizontal lines indicate the grand mean of ELISPOT responses to each antigen used for stimulation. Different tissue types (PBMC, MesLN, spleen) are indicated with individual symbols as shown. For specific tissue samples in which positive T cell responses to viral antigens were measured, the animal number is indicated next to the symbol. Group colors are consistent throughout the manuscript. Source data are provided as a Source Data file.

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