Fig. 5: Deletion of HuR in BAT promotes brown adipogenesis.
From: The RNA-binding protein HuR is a negative regulator in adipogenesis
a GSEA on HuR-FKO and Ctrl BAT indicated that myogenesis pathway was significantly downregulated in HuR knockout BAT. b Real-time PCR to confirm the expression of BAT-selective markers, adipogenesis, lipogenesis (Ctrl, n = 6; HuR-FKO, n = 7), and c muscle markers (Ctrl, n = 6; HuR-FKO, n = 8). d Western blot to exam the protein level of Ucp1, Pgc1a, and Desmin in BAT from HuR-FKO and Ctrl mice. e Western blot analysis of HuR expression in BAT, iWAT, and eWAT of HuR-BATKO and control mice. f Morphological characteristics of HuR-BATKO and control littermates by H&E staining. Scale bars represent 100 μM. g Real-time PCR to confirm the expression of genes for BAT-selective markers and adipogenesis in BAT from HuR-BATKO and Ctrl mice (Ctrl, n = 6; HuR-BATKO, n = 7). Error bars are mean ± SEM. Statistical significance was determined by Student’s t-test; *p < 0.05. Source data are provided as a Source Data file.
